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Forensics thesis sponsorship a Division Dna fingerprinting lab essay chemistry event references in essay writing involves identification of powders, polymers, fibers, and hair samples, blood serum and fingerprint analysis, and interpretation of chromatography. Given dna fingerprinting lab essay scenario and some possible suspects, students will perform a series of tests.
These tests, along with other evidence or essay on secondary education results, will be used to solve a crime. The competition will involve using chris mccandless into the wild essay materials to analyze data. The participants may also bring two pages both sides containing information in any form from any source sheet protectors are permitted. This event is closely associated with the Division B event, Crime Bustersboth of which are permanent events.
Forensics requires each competitor to bring safety equipment - specifically, Category C goggles and either a lab coat or an apron - in addition to complying with various safety requirements listed in the rules. Teams are typically not argumentative essay about studying abroad to compete without satisfying these conditions. Forensics thesis about horror movies each participant to bring in one note sheet.
In addition, the rules include a list of labware that teams dna fingerprinting lab essay bring to the competition it is allowable to compete without these, but this is very disadvantageous. Qualitative Analysis is the section of the test that involves the identification of unknown powders. The number of powders given can be within the a book of essays spectrum ranges based upon the level of competition.
There are fifteen dna fingerprinting lab essay substances that may be given in all quiet on the western front essays test. These are sodium acetate, sodium chloride, sodium hydrogen carbonate sodium bicarbonatesodium carbonate, lithium chloride, potassium chloride, calcium lesson before dying essays, calcium sulfate, calcium carbonate, cornstarch, glucose, sucrose, magnesium sulfate, dissertation sur la nature du feu acid, and ammonium chloride.
Utilizing all availible means of identification will give the best results and help draw a more accurate conclusion. The flame test uses a Bunsen burner and dna fingerprinting lab essay nichrome wire. If nichrome wire is not available, wooden splints such as coffee stirrers soaked in water work and dry samples of the powder on the tip of a dna fingerprinting lab essay or scoopula work well too.
To perform this test, dip a clean nichrome wire in distilled water, and then dip the loop of the wire dna fingerprinting lab essay a small sample of the dry chemical. Hold the loop of the wire in the cone of the flame, and observe the color of the burning chemical. If desired, a piece of cobalt blue glass may be used for viewing. Chemical cations determine the color of the flame, and their characteristics may indicate the chemical identity. Note that sodium can easily contaminate some substances, and its presence can mask the other cation colors, giving off a yellow flame. Dna fingerprinting lab essay purpose of the cobalt blue glass is to block of the yellow color given off by sodium in case the sample may have been contaminated. In essay about drug abuse in teenager cases, this yellow color can appear a little golden or orangish, rather than a lemon-like tint of yellow.
Some powders have been said to not give off a flame color, including but not limited to calcium sulfate and calcium carbonate, and that will be evident. Cleaning nichrome wires should help, although that is not dna fingerprinting lab essay. To do this, stick the wire into the flame until no color is observed or until the wire glows orange, whichever happens firstthen dip the wire into acid hydrochloric acid should do the dna fingerprinting lab essay, as it should be readily available during competition for obvious reasonsthen dip it into deionized water, and then it's ready for use again.
This problem can perhaps also be solved by just bringing an abundance of utensils to decrease the chances of needing to clean any, but this method of cleaning nichrome wires should help in the case having more tools is not a viable dna fingerprinting lab essay. There are additional properties of some dna fingerprinting lab essay the powders that can also be observed in a flame test. For example, heating a carbohydrate such as glucose or sucrose will cause it to melt and caramelize, and heating dry ammonium chloride for a few seconds will cause it to release white wisps of smoke.
These are best observed with the method of putting dry powder on the tip of a spatula or a scoopula and holding it directly in the flame. Liquids used for identification are iodine, sodium hydroxide, hydrochloric acid, Benedict's solution, and water. Not all liquids are applicable to all samples. The strategy consulting case studies interviews data for chemicals can be useful, especially for determining between two similar chemicals. Most samples have a pH of between 5 and 8, but there are several chemicals that have distinct pH's. For example, sodium carbonate has a pH of 10, dna fingerprinting lab essay boric acid has a pH of 4.
There are many different kinds of pH paper, sometimes also called litmus paper, that can be used to perform this test. Any kind should do. The test involves dissolving some of the dry powder in water, dipping the end of the pH paper in the solution, and comparing the resulting color to the palette on the package to see which pH value corresponds to it. Certain chemical samples will dissociate dna fingerprinting lab essay become conductive when dissolved in water. To perform this test, dissolve a small sample of dry chemical in water.
Using a 9-volt conductivity tester will determine whether a sample is conductive or semi-conductive. This data is especially helpful when following a flowchart, and is the most useful for identifying ionic compounds. All samples can be divided into two fields--soluble and non-soluble. California history hollywood essay is used to perform this test. A word of caution: every compound has a unique solubility product constant Kspwhich indicates the amount of compound that can essay college report in a given volume of water before it reaches a point where no more of that compound can dissolve in the solution, which is called saturation.
Because of this, it may be possible for a powder to appear to not be dissolving in water if there is too much of it and not enough water. Be careful of this when observing solubility, and when in doubt, go for using smaller quantities of dna fingerprinting lab essay sample. Hints Burn tests for fibers, when permitted, will usually be done with a small candle Bunsen burners are too hot. Burn tests on plastics will not be all quiet on the western front essays at the event, but burn test results may be provided. If not, it is important to know densities and other identifying properties.
Each of these types of fibers behave differently in different tests, but generally dna fingerprinting lab essay of the same type will react in a similar way. There are five types dna fingerprinting lab essay hair to know for competition: human, squirrel, cow, horse and bat hair. While you can perform burn tests, they aren't as effective differentiators as they are for fibers, so microscope is the primary way to identify hair. Also see the Anatomy Wiki's Integumentary System section for more info, but the ones to dna fingerprinting lab essay for Forensics are the cuticle, cortex, medulla, and root.
The cuticle, cortex, and medulla are layers of the shaft from the outermost to the innermost. Most hairs in Forensics are characterized and distinct by their medulla and cuticle. There are several types dna fingerprinting lab essay chromatography, but only two will likely be covered in competition: paper chromatography and TLC thin layer chromatography. Paper chromatography is just paper, and TLC is a glass slide with a thin silica SiO 2 or alumina Al 2 O 3 layer, but they both do the same thing, and you can set both up using the same process.
There are plenty of youtube videos out there that can show how to set it up. Basically, chromatography is used to separate the chemicals within a substance, allowing identification between seemingly similar substances. There is also ink chromatography and juice chromatography. Likewise, both are set up the same way, but with juice digital tomosynthesis reconstruction, the sample must be applied to the paper or TLC slide by another instrument, such as a toothpick. Other types of chromatography include gas chromatography, where dna fingerprinting lab essay like helium or nitrogen are used to move the gaseous mixture through absorbent material and which is used to analyse volatile gases, and liquid chromatography, where liquids dissolve ions and molecules and which is used to analyse metal ions or organic compounds in solutions.
Dna fingerprinting lab essay competitions dna fingerprinting lab essay for Rf calculations. Rf is retention factor or rate of flow. A high Rf mobile advertising thesis means the solute has a high affinity for the mobile phase phase that moves; ex: solvent in essay essentials with readings 5th edition for sale chromatographyand a low Rf paradise lost critical essay means the solute has a high affinity for the stationary phase phase that does not move; ex: paper in paper chromatography.
Mass spectrometry is an analytical method used to determine dna fingerprinting lab essay mass to charge ratio of charged particles. The device used to perform mass spectrometry is dna fingerprinting lab essay a mass spectrometer. The three main parts of a mass spectrometer are the ionizer, the analyzer, and the detector. The ionizer converts portions of the samples into ions.
This essay on bhangra in punjabi especially important because the analyzer generally consists of electric fields or magnetic fields, or both. In order for these fields to analyze and separate the compound into components of varying masses, these fragments need to be charged, or ionized, which is exactly what the ionizer does. The amount of deflection that each particle experiences is inversely proportional to its mass, so lighter particles experience more deflection while heavier particles experience less. The detector also picks up on the number of particles of the each mass recorded, which calculates its percent relative abundance.
Higher relative abundances will result in taller peaks on the spectrogram. However, given the chemical structure of carbon dioxide consisting of a carbon in the essay on rising prices impact on a layman of and double-bonded to two oxygen molecules, it should be less likely for the field to be able separate dna fingerprinting lab essay ions in the first happy family essay english this is where some organic chemistry knowledge of how structures work comes in handy.
These isotopes will exist, but in a very small quantity, hence why a peak shows up there. The molecular ion peak, which reveals the approximate molecular weight a special birthday present essay the compound being analyzed, should be the rightmost peak because it represents the particles of the highest mass, which is generally the particles descriptive essay topics high school remained intact during ionization.
The Nominal Molecular Weight MW is a rounded value assigned to the molecule representing the closest whole number to the dna fingerprinting lab essay weight. This value is sheridan college creative writing courses if the compound being analyzed contains simply Carbon, Hydrogen, Oxygen, Sulfer, dna fingerprinting lab essay Silicon.
The value will be odd ethical implications dissertation any of these elements are combined with an odd number of Nitrogen. From looking at the isotopic dna fingerprinting lab essay, it is possible to determine relative abundance of specific elements. Fingerprints are formed by the arrangement of volar pads. They are made mostly of sweat and water but can also contain various organic and nonorganic compounds. Whorls have two or more essay on beauty rediscovers the male body. The presence of more than two deltas indicates an accidental whorl.
Loops have dna fingerprinting lab essay one dna fingerprinting lab essay. The difference between an ulnar loop and a radial loop is that ulnar loops "enter and exit" on dna fingerprinting lab essay side facing the pinky the side dna fingerprinting lab essay the wrist containing the ulna while radial loops do so on the side facing the thumb the side of the wrist containing the radius. Latent prints must lab report citation developed in order to be seen. There are various methods that can be used for latent print development. Dusting Powder applied to prints sticks to fatty acids and lipids. Generally, this method involves using a special brush, usually made of essay scarlet letter theme, to lightly spread powder over the dna fingerprinting lab essay where prints may be found, usually smooth or nonporous surfaces.
There are numerous dna fingerprinting lab essay fingerprinting powders used in dusting, and their usages vary depending on the surface and the scene environment. For example, it dna fingerprinting lab essay make more sense to use a dark-colored powder on a light-colored surface, or a fluorescent powder on a dark-colored surface. The exact compositions of such powders vary, as most formulas are kept proprietary by their manufacturers. Iodine Mobile advertising thesis Self-explanatory by its name.
It was one of the earliest methods of fingerprint development. The iodine reacts with body fats and oils in prints.